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. 2013 Aug;89(2):376-9.
doi: 10.4269/ajtmh.13-0113. Epub 2013 Jun 24.

Molecular evidence of Trichostrongylus colubriformis and Trichostrongylus axei infections in humans from Thailand and Lao PDR

Issarapong Phosuk  1 , Pewpan M Intapan, Oranuch Sanpool, Penchom Janwan, Tongjit Thanchomnang, Kittisak Sawanyawisuth, Nimit Morakote, Wanchai Maleewong
Affiliations

Molecular evidence of Trichostrongylus colubriformis and Trichostrongylus axei infections in humans from Thailand and Lao PDR

Issarapong Phosuk et al. Am J Trop Med Hyg. 2013 Aug.

Abstract

Human trichostrongylosis has been reported in Thailand. Recent reports in Lao People's Democratic Republic concerning species identification urged us to investigate species distribution in Thailand. We report eight human cases in Thailand and Lao People's Democratic Republic that were found to be infected by Trichostrongylus colubriformis and T. axei identified and confirmed by molecular techniques. This evidence is the first molecular evidence of human T. colubriformis and T. axei infection in Thailand. Infection by these two species was apparently epidemic in these areas. It is necessary to proceed with more comprehensive veterinary and epidemiologic studies to enable the practical prevention and control of this parasitic zoonosis.

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Figures

Figure 1.
Figure 1.
(A) Distribution of trichostrongylosis cases in Thailand: (1) Khon Kaen, (2) Roi Et, (3) Loei, and (4 and 5) Sakon Nakhon. Distribution of trichostrongylosis cases in Lao PDR: (6) Bolikhamxai, (7) Khammouan, and (8) Savannakhet. (B) Egg of Trichostrongylus from patient's feces under the microscope. (C) Third-stage larva of Trichostrongylus from agar plate culture. Scale bar: 50 μm.
Figure 2.
Figure 2.
Phylogenetic trees of Trichostrongylus spp. were constructed by the neighbor-joining method with MEGA version 3.1. The left tree was from ITS1 gene sequences of Trichostrongylus spp., and the right tree was from ITS2 gene sequences. The sequences were deposited in GenBank, and bootstrap scores expressed as percentages of 1,000 replications are given at each node. Others from DNA database are indicated with species and accession numbers. The numbers of cases from the present study are indicated with human numbers 1–8 as follows: Thailand: Khon Kaen (human 1), Roi Et (human 2), Loei (human 3), and Sakon Nakhon (humans 4 and 5); Lao PDR: Bolikhamxai (human 6), Khammouan (human 7), and Savannakhet (human 8). Thailand (THA) and Lao PDR (LAO) are represented with country codes (ISO 3166-1 α-3 codes).
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