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. 2013 Apr;5(2):109-12.
doi: 10.4103/0974-8490.110540.

The triterpenoid fraction from Trichosanthes dioica root exhibits in vitro antileishmanial effect against Leishmania donovani promastigotes

Sanjib Bhattacharya  1 , Moulisha Biswas, Pallab K Haldar
Affiliations

The triterpenoid fraction from Trichosanthes dioica root exhibits in vitro antileishmanial effect against Leishmania donovani promastigotes

Sanjib Bhattacharya et al. Pharmacognosy Res. 2013 Apr.

Abstract

Background: Trichosanthes dioica Roxb. (Cucurbitaceae), called pointed gourd in English is a dioecious climber found wild throughout the plains of the Indian subcontinent and traditionally used in India for several medicinal purposes.

Objective: The present study was aimed at the evaluation of in vitro antileishmanial effect of triterpenoid fraction from T. dioica root (CETD).

Materials and methods: The antileishmanial activity of CETD was evaluated against Leishmania donovani (strain MHOM/IN/83/AG83)) promastigotes by in vitro promastigote cell toxicity assay by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide). Potassium antimonyl tartrate was used as reference.

Results: Here, CETD markedly inhibited the growth of L. donovani promastigotes in vitro in a concentration dependent manner and demonstrated IC50 value of 18.75 μg/ml. The reference drug potassium antimonyl tartrate exhibited IC50 of 7.52 μg/ml.

Conclusion: From the present study it can be inferred that the triterpenoid fraction of T. dioica root exhibited remarkable antileishmanial activity against Leishmania donovani promastigotes in vitro.

Keywords: Antileishmanial; Leishmania donovani; Trichosanthes dioica; cucurbitacins; promastigotes; root.

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Conflict of interest statement

Conflict of Interest: None declared

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