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Comment
. 2013 Jul 15;12(14):2173-4.
doi: 10.4161/cc.25551.

3D culture adds an extra dimension to targeted epigenetic therapies

Ali R Ozes  1 , Kenneth P Nephew
Affiliations
Comment

3D culture adds an extra dimension to targeted epigenetic therapies

Ali R Ozes et al. Cell Cycle. .
No abstract available

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Figures

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Figure 1. Comparing the sensitivity of epithelial ovarian cancer cells to the histone methyltransferase inhibitor (HMTI) GSK343 in 2D (i.e., plastic, upper) and 3D (i.e., matrigel extracellular matrix, lower). No effect of GSK343 on ovarian cancer cell migration, invasion, or apoptosis was observed using 2D culture conditions, despite the fact that the drug significantly reduced H3K27me3 levels. In 3D culture, however, GSK343 significantly inhibited ovarian cancer cell invasion and reduced cell survival, which was correlated with apoptosis induction (indicated by brown cells). The third dimension showcased the association of altered pathways (possibly by dysregulation of cellular proteins involved in ECM communication) with decreased in EZH2 activity.
See this image and copyright information in PMC

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