Comparative Study
doi: 10.1128/jcm.28.7.1669-1670.1990.
Enhancing effect of centrifugation on isolation of influenza virus from clinical specimens
Affiliations
- PMID: 2380390
- PMCID: PMC268014
- DOI: 10.1128/jcm.28.7.1669-1670.1990
Item in Clipboard
Comparative Study
Enhancing effect of centrifugation on isolation of influenza virus from clinical specimens
J Clin Microbiol.
1990 Jul.
Abstract
The use of centrifugation (700 x g, 60 min) in a plaque assay markedly increased (mean, 2.9-fold) the infectivity of all 42 influenza virus strains tested, compared with no centrifugation. Of 13 influenza virus strains isolated from 390 clinical specimens, 9 (69%) were efficiently isolated by the centrifugation assay compared with conventional culture methods. The centrifugation assay may be useful for isolating the influenza virus from clinical specimens.
Similar articles
-
Physical and chemical methods for enhancing rapid detection of viruses and other agents.Clin Microbiol Rev. 1993 Apr;6(2):150-75. doi: 10.1128/CMR.6.2.150. Clin Microbiol Rev. 1993. PMID: 8472247 Free PMC article. Review.
-
Enhanced isolation of influenza virus in conventional plate cell cultures by using low-speed centrifugation from clinical specimens.Am J Clin Pathol. 1991 Jun;95(6):765-8. doi: 10.1093/ajcp/95.6.765. Am J Clin Pathol. 1991. PMID: 2042583
-
Comparison of Influenza Virus Particle Purification Using Magnetic Sulfated Cellulose Particles with an Established Centrifugation Method for Analytics.Anal Chem. 2015 Nov 3;87(21):10708-11. doi: 10.1021/acs.analchem.5b02681. Epub 2015 Oct 14. Anal Chem. 2015. PMID: 26447768
-
Comparison between three rapid methods for direct diagnosis of influenza and the conventional isolation procedure.Biologicals. 1991 Oct;19(4):287-92. doi: 10.1016/s1045-1056(05)80017-x. Biologicals. 1991. PMID: 1797040
-
Selected laboratory aspects of influenza surveillance.Yale J Biol Med. 1982 May-Aug;55(3-4):201-5. Yale J Biol Med. 1982. PMID: 6758369 Free PMC article. Review.
Cited by
-
New aspects of influenza viruses.Clin Microbiol Rev. 1992 Jan;5(1):74-92. doi: 10.1128/CMR.5.1.74. Clin Microbiol Rev. 1992. PMID: 1310439 Free PMC article. Review.
-
Epidemiology of herpes simplex virus types 1 and 2 in Germany: what has changed?Med Microbiol Immunol. 2003 Aug;192(3):177-81. doi: 10.1007/s00430-003-0183-0. Epub 2003 May 22. Med Microbiol Immunol. 2003. PMID: 12761673
-
Physical and chemical methods for enhancing rapid detection of viruses and other agents.Clin Microbiol Rev. 1993 Apr;6(2):150-75. doi: 10.1128/CMR.6.2.150. Clin Microbiol Rev. 1993. PMID: 8472247 Free PMC article. Review.
-
Comparison of Madin-Darby canine kidney cells (MDCK) with a green monkey continuous cell line (Vero) and human lung embryonated cells (MRC-5) in the isolation of influenza A virus from nasopharyngeal aspirates by shell vial culture.J Clin Microbiol. 1997 Jul;35(7):1900-1. doi: 10.1128/jcm.35.7.1900-1901.1997. J Clin Microbiol. 1997. PMID: 9196221 Free PMC article.
-
Enhancement of feline infectious peritonitis virus type I infection in cell cultures using low-speed centrifugation.J Virol Methods. 1995 Feb;51(2-3):357-62. doi: 10.1016/0166-0934(94)00119-2. J Virol Methods. 1995. PMID: 7738156 Free PMC article.
References
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
