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. 2013 Jun 21;8(6):e67992.
doi: 10.1371/journal.pone.0067992. Print 2013.

A Single Nucleotide Polymorphism within the Interferon Gamma Receptor 2 Gene Perfectly Coincides with Polledness in Holstein Cattle

Sabrina Glatzer  1 , Nina Johanna Merten, Claudia Dierks, Anne Wöhlke, Ute Philipp, Ottmar Distl
Affiliations

A Single Nucleotide Polymorphism within the Interferon Gamma Receptor 2 Gene Perfectly Coincides with Polledness in Holstein Cattle

Sabrina Glatzer et al. PLoS One. .

Abstract

Polledness is a high impact trait in modern milk and beef production to meet the demands of animal welfare and work safety. Previous studies have mapped the polled-locus to the proximal region of the bovine chromosome 1 (BTA1) and narrowed it down to approximately 1 Mb. Sequencing of the positional candidate genes within the 1 Mb polled region and whole genome sequencing of Holsteins revealed a single nucleotide polymorphism (SNP) AC000158: g.1390292G>A within intron 3 of the interferon gamma receptor 2 gene (IFNGR2) in perfect co-segregation with polledness in Holsteins. This complete association was validated in 443 animals of the same breed. This SNP allows reliable genotyping of horned, heterozygous and homozygous polled Holsteins, even in animals that could not be resolved using the previously published haplotype for Holstein.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Gene structure of IFNGR2.
Rectangles indicate exons. Coding sequence is filled with black, the 3’ UTR is filled with white. The size of exons and introns is specified in number of base pairs (bp). The black line below illustrates the sections analysed by PCR in Holsteins. Each PCR-product is pictured separately with start and end position. Position of start and stop codon is stated in bp. All detected exonic polymorphisms and intronic polymorphisms showing association with polledness in Holsteins are given with name and position above the gene model. Each position is given with accordance to Bos taurus assembly UMD3.1.
Figure 2
Figure 2. Linkage disequilibrium among markers within the 1 Mb BTA1 region associated with polledness in Holsteins.
AC000158: g.1390292G>A, AC000158: g.1394768_771delCTGC and AC000158: g.1400851_852delCT are newly discovered polymorphisms located in the IFNGR2 gene. P5ID, PC1768587A and P80kbID represent three markers of the polled-haplotype previously published for Holstein [15]. LD coefficients (r 2) between pairs of polymorphisms are indicated as black letters in red fields. All pictured polymorphisms share one common haplotype block of 519 kb length.
See this image and copyright information in PMC

References

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