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. 2013:3:2104.
doi: 10.1038/srep02104.

In vivo and real-time monitoring of secondary metabolites of living organisms by mass spectrometry

Bin Hu  1 , Lei Wang, Wen-Cai Ye, Zhong-Ping Yao
Affiliations

In vivo and real-time monitoring of secondary metabolites of living organisms by mass spectrometry

Bin Hu et al. Sci Rep. 2013.

Abstract

Secondary metabolites are compounds that are important for the survival and propagation of animals and plants. Our current understanding on the roles and secretion mechanism of secondary metabolites is limited by the existing techniques that typically cannot provide transient and dynamic information about the metabolic processes. In this manuscript, by detecting venoms secreted by living scorpion and toad upon attack and variation of alkaloids in living Catharanthus roseus upon stimulation, which represent three different sampling methods for living organisms, we demonstrated that in vivo and real-time monitoring of secondary metabolites released from living animals and plants could be readily achieved by using field-induced direct ionization mass spectrometry.

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Figures

Figure 1
Figure 1. Photo of in vivo analysis of a living scorpion by field-induced DI-MS.
Figure 2
Figure 2. Mass spectrum obtained by field-induced DI-MS analysis of the secretion released from a living scorpion upon stimulation.
Figure 3
Figure 3. Mass spectrum obtained by field-induced DI-MS analysis of the secretion released from a living toad upon stimulation.
Figure 4
Figure 4. Field-induced DI spectra obtained for a leaf of a living C. rosues (a) before and (b) after heating.
Methanol (2 μL) was used as the added solvent.
See this image and copyright information in PMC

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