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. 2013;14(3):241-8.
doi: 10.4142/jvs.2013.14.3.241. Epub 2013 Jun 30.

GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum

Hun-Su Seo  1 , Kyoung Hee Kim, Dae-Yong Kim, Bong-Kyun Park, Nam-Shik Shin, Jae-Hoon Kim, Heejeong Youn
Affiliations

GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum

Hun-Su Seo et al. J Vet Sci. 2013.

Abstract

We analyzed alcoholic extracts of herbs possessing anti-neosporal activity against Neospora (N.) caninum. To identify the chemical components of Sophora (S.) flavescens and Torilis (T.) japonica associated with anti-neosporal activity, specific fractions were isolated by high-performance liquid chromatography (HPLC). In vitro activity of the fractions against N. caninum was then assessed. Gas chromatography/ mass spectrometry (GC/MS) was used to identify and quantify specific anti-neosporal molecules in the herbal extracts. Almost all HPLC fractions of S. flavescens and T. japonica had higher levels of anti-neosporal activity compared to the not treated control. Active constituents of the extracts were sophoridane, furosardonin A, and tetraisopropylidene-cyclobutane in S. flavescens; 5,17-β-dihydroxy-de-A-estra-5,7,9,14-tetraene, furanodiene, and 9,12-octadecadienoic acid (Z,Z)-(CAS,1) in T. japonica.

Keywords: GC/MS; HPLC; Neospora caninum; Sophora flavescens; Torilis japonica.

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Figures

Fig. 1
Fig. 1
HPLC fraction G1-4-1 (Sf-1) was identified as sophoridane.
Fig. 2
Fig. 2
HPLC fraction G3-1 (Sf-3) was identified as furosardonin A.
Fig. 3
Fig. 3
HPLC fraction G3-5 (Sf-4) was identified as tetraisopropylidene-cyclobutane.
Fig. 4
Fig. 4
HPLC fraction G3-6-1 (Tj-1) was identified as 5,17-β-dihydroxy-de-A-estra-5,7,9,14-tetraene.
Fig. 5
Fig. 5
HPLC fraction G4-6 (Tj-2) was identified as furanodiene.
Fig. 6
Fig. 6
HPLC fraction G5-2 (Tj-3) was identified as 9,12-octadecadienoic acid (Z,Z)-(CAS,1).
See this image and copyright information in PMC

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