Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Jul 2;14(7):13727-47.
doi: 10.3390/ijms140713727.

The organization of the quorum sensing luxI/R family genes in Burkholderia

Kumari Sonal Choudhary  1 , Sanjarbek Hudaiberdiev, Zsolt Gelencsér, Bruna Gonçalves Coutinho, Vittorio Venturi, Sándor Pongor
Affiliations

The organization of the quorum sensing luxI/R family genes in Burkholderia

Kumari Sonal Choudhary et al. Int J Mol Sci. .

Abstract

Members of the Burkholderia genus of Proteobacteria are capable of living freely in the environment and can also colonize human, animal and plant hosts. Certain members are considered to be clinically important from both medical and veterinary perspectives and furthermore may be important modulators of the rhizosphere. Quorum sensing via N-acyl homoserine lactone signals (AHL QS) is present in almost all Burkholderia species and is thought to play important roles in lifestyle changes such as colonization and niche invasion. Here we present a census of AHL QS genes retrieved from public databases and indicate that the local arrangement (topology) of QS genes, their location within chromosomes and their gene neighborhoods show characteristic patterns that differ between the known Burkholderia clades. In sequence phylogenies, AHL QS genes seem to cluster according to the local gene topology rather than according to the species, which suggests that the basic topology types were present prior to the appearance of current Burkholderia species. The data are available at http://net.icgeb.org/burkholderia/.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Clustering of LuxI protein sequences and perceived signals by LuxR homologues in complete Burkholderia genomes. In the following parts we review the AHL systems in the major Burkholderia clades.
Figure 2
Figure 2
QS regulatory circuits in Burkholderia cenocepacia J2315 [54].
Figure 3
Figure 3
Cladogram of the orthologs of ORF BCAM1871, encoding a protein with an HMG-CoA domain in Burkholderia spp. The orthologs are well separated into cepacia and pseudomallei groups. The RMI (R←M→I→) motive is flanked by conserved genes on both sides in members of both cepacia and pseudomallei groups. The numbers on the tree branches indicate bootstrap values (%).
Figure 4
Figure 4
Typical arrangements of QS genes in chromosome II of the Burkholderia cepacia complex (BCC) group. (A) Arrangement of QS genes in the BCC members with cciR/I genes (for example B. cenocepacia J2315 and B. cenocepacia MC0-3); (B) Arrangement of QS genes in BCC members having just one pair of luxR/I homologs (cepR/I) (for example B. cenocepacia AU1054, B. cenocepacia HI2424, B. cepacia GG4, etc.). OriC denotes the origin of replication, solo luxR genes (i.e. those without adjacent I genes) are denoted by black and white ovals, respectively, with the latter indicating two adjacent luxR homologues.
Figure 5
Figure 5
An example of the chromosomal arrangement of QS genes in completely sequenced genome of B. pseudomallei strains (for example B. pseudomallei 1026b, B. pseudomallei 1026a etc.). RXR: Two solo luxR homologs are separated by a hypothetical gene. The X gene is missing in some B. pseudomallei.
Figure 6
Figure 6
Schematic representation of the complex regulatory circuit in B. pseudomallei K92643 [61]. Dashed lines indicate partial regulation.
Figure 7
Figure 7
Regulatory circuit of BraR/I system in the plant-beneficial Burkholderia group (for example B. xenovorans LB400) [63].
Figure 8
Figure 8
Chromosomal arrangement of QS genes (braI/R) and OriC in completely sequenced members of the plant-beneficial and environmental group (for example, B. phytofirmans PsJN, B. xenovorans LB400, etc.).
Figure 9
Figure 9
Regulatory circuit of xenI2/R2 and bxeR genes in plant-beneficial and environmental group (for example, B. xenovorans LB400) [63].
See this image and copyright information in PMC

References

    1. Palleroni N.J., Kunisawa R., Contopoulou R., Doudoroff M. Nucleic Acid Homologies in the Genus Pseudomonas. Int. J. Syst. Bacteriol. 1973;23:333–339.
    1. Gillis M., Van T., Bardin R., Goor M., Hebbar P., Willems A., Segers P., Kersters K., Heulin T., Fernandez M.P. Polyphasic Taxonomy in the Genus Burkholderia Leading to an Emended Description of the Genus and Proposition of Burkholderia vietnamiensis sp. nov. for N2-Fixing Isolates from Rice in Vietnam. Int. J. Syst. Bacteriol. 1995;45:274–289.
    1. Yabuuchi E., Kosako Y., Oyaizu H., Yano I., Hotta H., Hashimoto Y., Ezaki T., Arakawa M. Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol. Immunol. 1992;36:1251–1275. - PubMed
    1. Isles A., Maclusky I., Corey M., Gold R., Prober C., Fleming P., Levison H. Pseudomonas cepacia infection in cystic fibrosis: An emerging problem. J. Pediatr. 1984;104:206–210. - PubMed
    1. Vanlaere E., Lipuma J.J., Baldwin A., Henry D., De Brandt E., Mahenthiralingam E., Speert D., Dowson C., Vandamme P. Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex. Int. J. Syst. Evolut. Microbiol. 2008;58:1580–1590. - PubMed

Publication types