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. 2013 Sep;20(9):1479-82.
doi: 10.1128/CVI.00375-13. Epub 2013 Jul 3.

Modification of clearview tuberculosis (TB) enzyme-linked immunosorbent assay for TB patients not infected with HIV

Laura Savolainen  1 , Anu Kantele, Bettina Sandboge, Marita Sirén, Heikki Valleala, Riitta Tuompo, Liana Pusa, Riitta Erkinjuntti-Pekkanen, Aija Knuuttila, Cheng-Lung Ku, Chih-Yu Chi, Tuula Vasankari, Tamara Tuuminen
Affiliations

Modification of clearview tuberculosis (TB) enzyme-linked immunosorbent assay for TB patients not infected with HIV

Laura Savolainen et al. Clin Vaccine Immunol. 2013 Sep.

Abstract

Diagnosis of active tuberculosis by detection of urinary lipoarabinomannan (uLAM) from Mycobacterium tuberculosis is an attractive approach. Concentrating urine 100-fold allowed quantitation of uLAM at levels equal to picograms/ml of nonconcentrated urine. The approach of concentrating urine 100-fold improved the clinical sensitivity of the Clearview TB enzyme-linked immunosorbent assay (ELISA) from 7% to 57% yet impaired its specificity from 97% to 89%. (This study has been registered at University Hospital of Turku under registration no. 47/180/2009, Helsinki University Central Hospital under 149/2010, University Hospital of Kuopio under 105/2010, and China Medical University Hospital, Taichung, under DMR-99-IRB-075-2.).

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Figures

Fig 1
Fig 1
Analysis of urine samples from patient groups and healthy volunteers. ODs of nonconcentrated (A) and concentrated (B) samples from healthy (n = 101), PF-TB (n = 28), and EPF-TB (n = 7) groups. (C) Calibration curve of uLAM samples (range, 0 to 20 ng/ml, nonconcentrated). (D) uLAM concentrations estimated from the calibration curve. Healthy (n = 101), PF-TB (n = 28), EPF-TB (n = 7), and LTBI (n = 15) groups. Horizontal bars show the mean concentrations. The cutoff (1.1 ng/ml) is presented with a dashed line.
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