Roles of estrogen and progesterone in modulating renal nerve function in the rat kidney

Abstract

The maintenance of extracellular Na+ and Cl- concentrations in mammals depends, at least in part, on renal function. It has been shown that neural and endocrine mechanisms regulate extracellular fluid volume and transport of electrolytes along nephrons. Studies of sex hormones and renal nerves suggested that sex hormones modulate renal function, although this relationship is not well understood in the kidney. To better understand the role of these hormones on the effects that renal nerves have on Na+ and Cl- reabsorption, we studied the effects of renal denervation and oophorectomy in female rats. Oophorectomized (OVX) rats received 17β-estradiol benzoate (OVE, 2.0 mg · kg(-1) · day(-1), sc) and progesterone (OVP, 1.7 mg · kg(-1) · day(-1), sc). We assessed Na+ and Cl- fractional excretion (FENa+ and FECl- , respectively) and renal and plasma catecholamine release concentrations. FENa+ , FECl- , water intake, urinary flow, and renal and plasma catecholamine release levels increased in OVX vs control rats. These effects were reversed by 17β-estradiol benzoate but not by progesterone. Renal denervation did not alter FENa+ , FECl- , water intake, or urinary flow values vs controls. However, the renal catecholamine release level was decreased in the OVP (236.6 ± 36.1 ng/g) and denervated rat groups (D: 102.1 ± 15.7; ODE: 108.7 ± 23.2; ODP: 101.1 ± 22.1 ng/g). Furthermore, combining OVX + D (OD: 111.9 ± 25.4) decreased renal catecholamine release levels compared to either treatment alone. OVE normalized and OVP reduced renal catecholamine release levels, and the effects on plasma catecholamine release levels were reversed by ODE and ODP replacement in OD. These data suggest that progesterone may influence catecholamine release levels by renal innervation and that there are complex interactions among renal nerves, estrogen, and progesterone in the modulation of renal function.

Figure 1. Renal catecholamine content in female rats. A, Control (C), ovariectomized (OVX), OVX with 17β-estradiol benzoate (OVE, 2.0 mg·kg^-1·day^-1, sc), and OVX with progesterone (OVP, 1.7 mg·kg^-1·day^-1, sc), both for 7 days. B, Sham-denervated (CD), denervated (D), D ovariectomized (OD), OD with 17β-estradiol benzoate (ODE), and OD with progesterone (ODP), both for 7 days. Data are reported as means±SE (n=8). *P<0.01 and ⁺P<0.01 compared to C and CD, respectively (ANOVA and Tukey test).

Figure 2. Plasma catecholamine levels in female rats. A, Control (C), ovariectomized (OVX), OVX with 17β-estradiol benzoate (OVE, 2.0 mg·kg^-1·day^-1, sc), and OVX with progesterone (OVP, 1.7 mg·kg^-1·day^-1, sc), both for 7 days. B, Sham-denervated (CD), denervated (D), D ovariectomized (OD), OD with 17β-estradiol benzoate (ODE), and OD with progesterone (ODP), both for 7 days. Data are reported as means±SE (n=8). *P<0.01 and ⁺P<0.01 compared to C and CD, respectively (ANOVA and Tukey test).