Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Aug 19;587(16):2635-42.
doi: 10.1016/j.febslet.2013.06.048. Epub 2013 Jul 4.

NMR structure of the N-terminal-most HRDC1 domain of RecQ helicase from Deinococcus radiodurans

Shanshan Liu  1 , Wen Zhang, Zengqiang Gao, Qianqian Ming, Haifeng Hou, Wenxian Lan, Houming Wu, Chunyang Cao, Yuhui Dong
Affiliations
Free article

NMR structure of the N-terminal-most HRDC1 domain of RecQ helicase from Deinococcus radiodurans

Shanshan Liu et al. FEBS Lett. .
Free article

Abstract

The RecQ helicase from Deinococcus radiodurans (DrRecQ) distinguishes from other helicases in that it utilizes its three 'helicase and RNaseD C-terminal' domains (HRDC1, HRDC2 and HRDC3) to regulate its activity. These HRDC domains have different influence on the biochemical functions of DrRecQ. Currently, only the structure of HRDC3 was reported. Here, we determined the NMR structure of the N-terminal-most HRDC1, revealing a potential DNA binding domain. Fluorescence anisotropy assay indicates that HRDC1 has binding affinity weaker than 70 μM to all DNA substrates without any specificity. Biochemical assays suggested that HRDC1 cooperates with other domains to enhance full-length DrRecQ interactions with DNA.

Keywords: DNA; DrRecQ; HRDC1; NMR; Structure.

PubMed Disclaimer

Publication types

LinkOut - more resources