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. 2013 Aug;10(8):737-40.
doi: 10.1038/nmeth.2556. Epub 2013 Jul 7.

Nanoscopy with more than 100,000 'doughnuts'

Andriy Chmyrov  1 , Jan Keller, Tim Grotjohann, Michael Ratz, Elisa d'Este, Stefan Jakobs, Christian Eggeling, Stefan W Hell
Affiliations

Nanoscopy with more than 100,000 'doughnuts'

Andriy Chmyrov et al. Nat Methods. 2013 Aug.

Abstract

We show that nanoscopy based on the principle called RESOLFT (reversible saturable optical fluorescence transitions) or nonlinear structured illumination can be effectively parallelized using two incoherently superimposed orthogonal standing light waves. The intensity minima of the resulting pattern act as 'doughnuts', providing isotropic resolution in the focal plane and making pattern rotation redundant. We super-resolved living cells in 120 μm × 100 μm-sized fields of view in <1 s using 116,000 such doughnuts.

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Comment in

  • Parallel super-resolution imaging.
    Rowlands CJ, Yew EY, So PT. Rowlands CJ, et al. Nat Methods. 2013 Aug;10(8):709-10. doi: 10.1038/nmeth.2567. Nat Methods. 2013. PMID: 23900251 Free PMC article.

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