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. 2013 Apr;4(2):94-100.
doi: 10.4103/2231-4040.111525.

Aqueous extract of Saussurea lappa root ameliorate oxidative myocardial injury induced by isoproterenol in rats

T S Mohamed Saleem  1 , N Lokanath, A Prasanthi, M Madhavi, G Mallika, M N Vishnu
Affiliations

Aqueous extract of Saussurea lappa root ameliorate oxidative myocardial injury induced by isoproterenol in rats

T S Mohamed Saleem et al. J Adv Pharm Technol Res. 2013 Apr.

Abstract

Saussurea lappa Clarke (Compositae), is commonly known as Kushta. In Ayurvedha, it is mentioned that the aqueous extract of the root S. lappa was used for treatment of angina pectoris. The present study was designed to investigate the cardioprotective effect of aqueous extract of root of S. lappa against isoproterenol induced myocardial injury. Myocardial injury in rat was induced by the administration of isoproterenol at a dose of 85 mg/kg, i.p., The rats were pretreated with the aqueous extract of S. lappa (AESL) in three different doses (100, 200 and 300 mg/kg, p.o.) through the oral route. Isoproterenol alone-treated rats showed increased serum concentration of lactate dehydrogenase (LDH), creatinine kinase (CK), and aspartate transaminase (AST), increased myocardial thiobarbituric acid reactive substances (TBARS) level, and decreased myocardial glutathione (GSH) level due to myocardial damage produced by isoproterenol. This is further conformed by histopathological changes. Chronic oral administration of AESL in three different doses significantly restored the level of myocardial LDH, CK, AST, TBARS, and GSH. The extract effect was compared with the reference standard α-tocopherol which also offered similar protection in biochemical and histopathological changes. The overall beneficial effect which was observed with the dose of 200 mg/kg indicated that AESL produced significant dose-dependent activity against isoproterenol induced myocardial injury.

Keywords: Antioxidants; Saussurea lappa; lactate dehydrogenase; myocardial infarction; oxidative stress.

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Conflict of interest statement

Conflict of Interest: Nil.

Figures

Figure 1
Figure 1
Reducing power of aqueous extract of Saussurea lappa
Figure 2
Figure 2
2,2-diphenyl-1-picrylhydrazyl inhibition activity of aqueous extract of Saussurea lappa
Figure 3
Figure 3
Hydrogen peroxide radical scavenging activity of aqueous extract of Saussurea lappa
Figure 4
Figure 4
Histopathological report. (a) Group I: Vehicle-received rat heart shows the normal cyto-architecture of the myocardium. (b) Group II: ISO-treated rat heart shows the necrotic changes in myocardial tissue. (c) Group III: Aqueous extract of Saussurea lappa 1 (100 mg/kg)-treated rat heart shows regenerative changes in myocardial tissue. (d) Group IV: Aqueous extract of Saussurea lappa 2 (200 mg/kg)-treated rat heart shows regenerative changes in myocardial tissue. (e) Group V: SL (300 mg/kg)-treated rat heart shows normal cyto-architecture of myocardium. (f) Group VI: α-tocopherol (60 mg/kg)-treated rat heart shows normal cyto-architecture of myocardium. N=Nucleus; ID=Intercalated disks; NC= Necrotic changes; MC= Myocardial cells; DC= Degenerative changes; RC= Regenerative changes
See this image and copyright information in PMC

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