Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2013 Jul;3(7):523-31.
doi: 10.1016/S2221-1691(13)60107-0.

Anti-trypanosomal effect of Peristrophe bicalyculata extract on Trypanosoma brucei brucei-infected rats

Abdulazeez Mansurah Abimbola  1 , Ibrahim Abdulrazak Baba, Edibo Zakari Yenusa, Sidali Joseph Omanibe, Idris Habeeb Oladimeji
Affiliations
Comparative Study

Anti-trypanosomal effect of Peristrophe bicalyculata extract on Trypanosoma brucei brucei-infected rats

Abdulazeez Mansurah Abimbola et al. Asian Pac J Trop Biomed. 2013 Jul.

Abstract

Objective: To investigate the in vitro and in vivo effect of whole plant extracts of Peristrophe bicalyculata on Trypanosoma brucei brucei-infected rats.

Methods: THE EXPERIMENT WAS DIVIDED INTO TWO PHASES: In the first phase, the anti-trypanosomal activity of the hot water, cold water, methanol and butanol extracts of the whole plant were determined by incubating with Trypanosoma brucei brucei. The cold water extract was partially-purified and the anti-trypanosomal activity of the fractions determined. In the second phase, Trypanosoma brucei brucei-infected rats were treated with fraction 2c for nine days. Packed cell volume (PCV), high density lipoprotein (HDL), low density lipoprotein (LDL), total cholesterol (TC), triacylglycerol (TAG), aspartate aminotransferase, alanine aminotransferases (ALT), alkaline phosphatase (ALP), total and direct bilirubin levels were determined at the end of the experiment.

Results: Cold water extract immobilized 90% of the parasites after 60 min of incubation, and fraction 2c completely immobilized the parasites after 35 min. It significantly increased PCV in Trypanosoma brucei brucei-infected rats. Decreased TC, TAG, HDL and LDL levels of infected rats increased significantly when rats were treated with the fraction, while elevated levels of total bilirubin and ALT also decreased. The difference in urea, direct bilirubin and ALP was not significant when infected rats were compared to rats in other groups.

Conclusions: The ability of the plant to ameliorate the infection-induced biochemical changes calls for detailed investigation of the potentials of the plant for antitrypanosomiasis drug delivery.

Keywords: Anti-trypanosomal activity; Lipoproteins; Peristrophe bicalyculata; Trypanosoma brucei brucei.

PubMed Disclaimer

Conflict of interest statement

Conflict of interest statement: We declare that we have no conflict of interest.

Figures

Figure 1.
Figure 1.. The in vitro anti-trypanosomal activity of cold extract of Peristrophe bicalyculata at different concentrations.
Figure 2.
Figure 2.. The in vitro anti-trypanosomal activity of hot water extract of Peristrophe bicalyculata at different concentrations.
Figure 3.
Figure 3.. The in vitro anti-trypanosomal effect of methanol extract of Peristrophe bicalyculata at different concentrations.
Figure 4.
Figure 4.. The in vitro anti-trypanosomal effect of butanol extract of Peristrophe bicalyculata at different concentrations.
Figure 5.
Figure 5.. The in vitro anti-trypanosomal activity of partially-purified extract of Peristrophe bicalyculata (fraction 1).
Figure 6.
Figure 6.. The in vitro anti-trypanosomal activity of partially-purified extract of Peristrophe bicalyculata (fraction 2a).
Figure 7.
Figure 7.. The in vitro anti-trypanosomal activity of partially-purified extract of Peristrophe bicalyculata (fraction 2c).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Denbarga Y, Ando O, Abebe R. Trypanosoma species causing bovine trypanosomosis in South Achefer District, Northern Ethiopia. J Vet Adv. 2012;2(2):108–113.
    1. Guilherme M, Andre T. Nutritional status driving infection by Trypanosoma cruzi: Lessons from experimental animals. J Trop Med. 2011;2011:981879. - PMC - PubMed
    1. Jackson AP, Berry A, Aslett M, Allison HC, Burton P, Anderson AV, et al. et al. Antigenic diversity is generated by distinct evolutionary mechanisms in African trypanosome species. Proc Natl Acad Sci USA. 2012;109(9):3416–3421. - PMC - PubMed
    1. Oduola T, Bello I, Adeosun G, Ademosun A, Raheem G, Avwioro G. Hepatotoxicity and nephrotoxicity evaluation in Wistar albino rats exposed to Morinda lucida leaf extract. N Am J Med Sci. 2010;2(5):230–233. - PMC - PubMed
    1. Devaki K, Beulah U, Akila G, Gopalakrishnan VK. Effect of aqueous extract of Passiflora edulis on biochemical and hematological parameters of Wistar albino rats. Toxicol Int. 2012;19(1):63–67. - PMC - PubMed

Publication types

LinkOut - more resources