doi: 10.1016/0014-5793(90)80991-q.
3,4-Dichloroisocoumarin, a serine protease inhibitor, inactivates glycogen phosphorylase b
Affiliations
- PMID: 2384149
- DOI: 10.1016/0014-5793(90)80991-q
Item in Clipboard
3,4-Dichloroisocoumarin, a serine protease inhibitor, inactivates glycogen phosphorylase b
FEBS Lett.
.
Free article
Abstract
3,4-Dichloroisocoumarin (3,4-DCI) is a highly reactive, mechanism-based inhibitor of serine proteases. We show here that glycogen phosphorylase b is also inactivated by this inhibitor, in a mechanism that parallels the inactivation of serine proteases, but involving multiple sites of covalent modification. Such a process may compromise studies in which 3,4-DCI is used to arrest proteolysis of a second native protein which may itself be modified.
Similar articles
-
The function of lymphocyte proteases. Inhibition and restoration of granule-mediated lysis with isocoumarin serine protease inhibitors.J Immunol. 1991 Aug 15;147(4):1360-8. J Immunol. 1991. PMID: 1869829
-
Apparent formation of sodium dodecyl sulfate-stable complexes between serpins and 3,4-dichloroisocoumarin-inactivated proteinases is due to regeneration of active proteinase from the inactivated enzyme.J Biol Chem. 1997 May 16;272(20):13338-42. doi: 10.1074/jbc.272.20.13338. J Biol Chem. 1997. PMID: 9148956
-
The susceptibility of glycogen phosphorylase to inactivation by endogenous and exogenous proteases.Acta Biol Med Ger. 1977;36(11-12):1637-44. Acta Biol Med Ger. 1977. PMID: 616710
-
Structure of 3,4-dichloroisocoumarin-inhibited factor D.Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):711-7. doi: 10.1107/s0907444997010457. Acta Crystallogr D Biol Crystallogr. 1998. PMID: 9757085
-
Mechanism-based isocoumarin inhibitors for serine proteases: use of active site structure and substrate specificity in inhibitor design.J Cell Biochem. 1989 Jan;39(1):33-46. doi: 10.1002/jcb.240390105. J Cell Biochem. 1989. PMID: 2654146 Review.
Cited by
-
Identification and Characterization of Approved Drugs and Drug-Like Compounds as Covalent Escherichia coli ClpP Inhibitors.Int J Mol Sci. 2019 May 31;20(11):2686. doi: 10.3390/ijms20112686. Int J Mol Sci. 2019. PMID: 31159170 Free PMC article.
-
Human preprocalcitonin self-antigen generates TAP-dependent and -independent epitopes triggering optimised T-cell responses toward immune-escaped tumours.Nat Commun. 2018 Nov 30;9(1):5097. doi: 10.1038/s41467-018-07603-1. Nat Commun. 2018. PMID: 30504837 Free PMC article.
-
Preprocalcitonin signal peptide generates a cytotoxic T lymphocyte-defined tumor epitope processed by a proteasome-independent pathway.Proc Natl Acad Sci U S A. 2008 Jul 22;105(29):10119-24. doi: 10.1073/pnas.0802753105. Epub 2008 Jul 14. Proc Natl Acad Sci U S A. 2008. PMID: 18626012 Free PMC article.
-
Inducible phosphorylation of I kappa B alpha is not sufficient for its dissociation from NF-kappa B and is inhibited by protease inhibitors.Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):11884-8. doi: 10.1073/pnas.91.25.11884. Proc Natl Acad Sci U S A. 1994. PMID: 7991551 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
