Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013:2013:219439.
doi: 10.1155/2013/219439. Epub 2013 Jun 13.

Recombinant varicella-zoster virus vaccines as platforms for expression of foreign antigens

Wayne L Gray  1
Affiliations

Recombinant varicella-zoster virus vaccines as platforms for expression of foreign antigens

Wayne L Gray. Adv Virol. 2013.

Abstract

Varicella-zoster virus (VZV) vaccines induce immunity against childhood chickenpox and against shingles in older adults. The safety, efficacy, and widespread use of VZV vaccines suggest that they may also be effective as recombinant vaccines against other infectious diseases that affect the young and the elderly. The generation of recombinant VZV vaccines and their evaluation in animal models are reviewed. The potential advantages and limitations of recombinant VZV vaccines are addressed.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Cosmid approach to generate rVZV. (a) The 125 kb VZV genome consists of a unique long (UL) component flanked by internal and terminal inverted repeat sequences (IRL and TRL) covalently linked to a unique short (US) component bracketed by internal and terminal inverted repeats (IRS and TRS) [17]. (b) Four cosmids (30–45 kb), representing the entire VZV genome, are transfected into MeWo cells yields infectious virus. In this example, a foreign gene with a promoter is inserted into cosmid A, within the intergenic region between VZV ORFs 12 and 13.
Figure 2
Figure 2
VZV-BAC approach to generate rVZV. BAC sequences are inserted into the VZV genome within the intergenic region between ORFs 11 and 12 and maintained within E. coli. A foreign gene of interest (goi) with promoter is inserted between ORFs 65 and 66. rVZV-BAC DNA is transfected into MeWo cells to generate infectious rVZV. BAC sequences flanked by lox P sites (∗) may be removed from the VZV genome by Cre recombination.
See this image and copyright information in PMC

References

    1. Cohen JI, Straus SE, Arvin AM. Varicella-zoster virus replication, pathogenesis, and management. In: Knipe DM, Howley PM, Griffin DE, et al., editors. Field's Virology. 5th edition. Philadelphia, Pa, USA: Lippincott Williams & Wilkins; 2007. pp. 2774–2840.
    1. Feldman S, Hughes WT, Daniel CB. Varicella in children with cancer: seventy seven cases. Pediatrics. 1975;56(3):388–397. - PubMed
    1. Wood SM, Shah SS, Steenhoff AP, Rutstein RM. Primary varicella and herpes zoster among HIV-infected children from 1989 to 2006. Pediatrics. 2008;121(1):e150–e156. - PubMed
    1. Shrim A, Koren G, Farine D, et al. Management of varicella infection (chickenpox) in pregnancy. Journal of the Obstetrics and Gynaecology Canada. 2012;34(3):287–292. - PubMed
    1. Gilden D, Mahalingam R, Nagel MA, Pugazhenthi S, Cohrs RJ. Review: the neurobiology of varicella zoster virus infection. Neuropathology and Applied Neurobiology. 2011;37(5):441–463. - PMC - PubMed