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Comparative Study
. 2013 Jul;27(4):284-9.
doi: 10.1002/jcla.21598.

Measurement of serum total vitamin D (25-OH) using automated immunoassay in comparison [corrected] with liquid chromatography tandem-mass spectrometry

Sang-Yong Shin  1 , Min-Jung Kwon, Junghan Song, Hyosoon Park, Hee-Yeon Woo
Affiliations
Comparative Study

Measurement of serum total vitamin D (25-OH) using automated immunoassay in comparison [corrected] with liquid chromatography tandem-mass spectrometry

Sang-Yong Shin et al. J Clin Lab Anal. 2013 Jul.

Erratum in

  • J Clin Lab Anal. 2013 Sep;27(5):340

Abstract

Background: The associations of vitamin D deficiency with many nonskeletal diseases are still being discovered. We evaluated the use of an automated immunoassay to measure serum total vitamin D (25-OH) and assessed vitamin D status in a Korean adult population.

Methods: We compared the Elecsys Vitamin D (25-OH) Total Assay (Roche Diagnostics) with liquid chromatography-tandem mass spectrometry (LC-MS/MS) using 300 serum samples. Total imprecision was calculated using three levels of quality control materials and serum samples. We also investigated the vitamin D status using data for 70,762 cases who had a routine health check-up in our hospitals.

Results: The regression equation: Elecsys = 0.882 × LC-MS/MS + 6.814 (r = 0.926). Total imprecision was within 10% for all quality control materials and serum samples. The prevalence of vitamin D deficiency using cut-off values of <50 nmol/l (<20 ng/ml) were 70.3% in males and 86.4% in females, respectively. The prevalence of vitamin D deficiency was higher in younger subjects than in older subjects (P for linear-by-linear association was <0.001). Serum vitamin D levels were highest in September and lowest in February.

Conclusion: The Elecsys Vitamin D (25-OH) Total Assay was comparable to LC-MS/MS and appropriate for routine clinical use. Vitamin D deficiency is common in Korean adults.

Keywords: LC-MS/MS; electrochemiluminescence; vitamin D.

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Figures

Figure 1
Figure 1
Comparison of Elecsys with LC‐MS/MS for vitamin D (25‐OH) total assay. Scatter plot (A) and bias plot (B) are shown. The dotted line indicates standard line (A) and zero bias line (B).
Figure 2
Figure 2
Prevalence of vitamin D insufficiency (50–75 nmol/l) and deficiency (<50 nmol/l) according to age group. The proportions of males and females with vitamin D level below 75 nmol/l were significantly decreased with age (P = 0.01 analyzed by linear‐by‐linear association).
Figure 3
Figure 3
Box and whisker plots of total vitamin D (25‐OH) concentration according to month of collection. Boxes show interquartile range and median values. Samples were not collected in July and August.
See this image and copyright information in PMC

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