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. 2013 Jul 15:13:320.
doi: 10.1186/1471-2334-13-320.

Changing profile of rotavirus genotypes in Bangladesh, 2006-2012

Mokibul Hassan Afrad  1 , Zahid Hassan, Saiada Farjana, Sayra Moni, Subarna Barua, Sumon Kumar Das, Abu Syed Golam Faruque, Tasnim Azim, Mustafizur Rahman
Affiliations

Changing profile of rotavirus genotypes in Bangladesh, 2006-2012

Mokibul Hassan Afrad et al. BMC Infect Dis. .

Abstract

Background: Rotavirus is the leading cause of severe diarrhea in infants and young children worldwide including Bangladesh. Unlike what was seen in high-income countries, the licensed rotavirus vaccines did not show high efficacy in Bangladeshi trials. We assessed rotavirus prevalence and genotypes in Bangladesh over six-year period to provide baseline information on the rotavirus burden and changing profile in the country.

Methods: This study was conducted from June 2006 to May 2012 in Matlab, Bangladesh. Group A rotaviruses were detected in stools collected from diarrhea patients by ELISA and genotyped using multiplex reverse transcription PCR followed by nucleotide sequencing.

Results: Of the 9678 stool samples, 20.3% were positive for rotavirus. The most predominant genotype was G1P[8] (22.4%), followed by G9P[8] (20.8%), G2P[4] (16.9%) and G12P[8] (10.4%). Mixed infections were detected in 14.2% of the samples. Emergence of an unusual strain, G9P[4] was documented during 2011-12. Several amino acid mismatches in the antigenic epitopes of VP7 and VP4 between Bangladeshi and the vaccine strains were identified.

Conclusions: Our study provides important information on rotavirus genotypes that should be considered for the selection and introduction of rotavirus vaccines in Bangladesh.

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Figures

Figure 1
Figure 1
Prevalence and genotype distribution of rotaviruses in Matlab, Bangladesh. A: Number of diarrhea patients attended and number of cases tested rotavirus positive, June 2001-May 2012. B: Temporal changes in the distribution of major rotavirus genotypes, June 2006-May 2012.
Figure 2
Figure 2
Amino acid substitutions among Bangladeshi rotavirus strains (G1, G2, G9, G12 and P[8]) and vaccine strains in RotaTeq™ (G1, G2, G3, G4 and P[8]) and Rotarix™ (G1 and P[8]). Differences in amino acids were calculated from the epitopic regions of the VP7 and VP4 proteins. BGD, Bangladesh.
Figure 3
Figure 3
Amino acid substitutions among Bangladeshi rotavirus strains (G1, G2, G9, G12 and P[8]) and vaccine strains in RotaTeq™ (G1, G2, G3, G4 and P[8]) and Rotarix™ (G1 and P[8]). Differences in amino acids were calculated from the epitopic regions of the VP7 and VP4 proteins. BGD, Bangladesh.
See this image and copyright information in PMC

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