Temporal expression patterns of rainbow trout immune-related genes in response to Myxobolus cerebralis exposure

Abstract

Infection of salmonids by the myxozoan parasite Myxobolus cerebralis can cause whirling disease, which is responsible for high mortalities in rainbow trout hatcheries and natural populations in the United States. Although considerable research has provided insight into disease pathology, host invasion, and inheritance patterns of resistance, the causal genetic variants and molecular mechanisms underlying host resistance or susceptibility remain elusive. A previous study found that expression changes of specific metallothionein genes following M. cerebralis infection are implicated in whirling disease resistance. The present study examines the dynamic transcriptional response to infection of several upstream regulators of the metallothionein gene family (IL-1β, KLF2, STAT3, STAT5), along with innate immune response genes (IFN-γ, IRF1 and iNOS). Pathogen loads and gene expression were compared across multiple time points after M. cerebralis exposure to elucidate how resistant and susceptible rainbow trout strains transcriptionally respond to early invasion. IL-1β, IFN-γ, IRF1, and iNOS all showed increased expression following M. cerebralis exposure for one or both strains across multiple time points. The interferon-related genes IFN-γ and IRF1 had consistently increased expression in the susceptible strain in comparison to the resistant strain, likely due to a less effective initial immune response. STAT3 was the only gene with consistently increased expression in the resistant strain following infection while remaining unchanged in the susceptible strain. Given its pleiotropic effects on immune response, STAT3 is an excellent candidate for future research of whirling disease resistance mechanisms.

Keywords: Myxobolus cerebralis; Myxozoan pathogen; Oncorhynchus mykiss; STAT3; Whirling disease.