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. 2013 Oct;77(2):126-8.
doi: 10.1016/j.diagmicrobio.2013.06.011. Epub 2013 Jul 23.

Performance of MALDI-ToF MS for species identification of Burkholderia cepacia complex clinical isolates

Lorena Cristina Corrêa Fehlberg  1 , Lucas Henrique Sales Andrade, Diego Magno Assis, Rosana Helena Vicente Pereira, Ana Cristina Gales, Elizabeth Andrade Marques
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Performance of MALDI-ToF MS for species identification of Burkholderia cepacia complex clinical isolates

Lorena Cristina Corrêa Fehlberg et al. Diagn Microbiol Infect Dis. 2013 Oct.

Abstract

We evaluated the performance of matrix-assisted laser desorption ionization-time of flight (MALDI-ToF) for identification of Bcc species compared with that of recA sequencing. MALDI-ToF was able of identifying 100% of Bcc isolates at the genus level, but 23.1% of Bcc isolates tested were not correctly identified at the species level. The misidentification occurred most frequently with Burkholderia contaminans (100%) and B. cepacia (33.3%).

Keywords: Bacteria identification; Bruker; Mass spectrometry; Non-fermentative bacilli; recA gene sequencing.

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