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. 2013 Jul 11:(77):e50449.
doi: 10.3791/50449.

Technical aspects of the mouse aortocaval fistula

Kota Yamamoto  1 , Xin Li, Chang Shu, Tetsuro Miyata, Alan Dardik
Affiliations

Technical aspects of the mouse aortocaval fistula

Kota Yamamoto et al. J Vis Exp. .

Abstract

Technical aspects of creating an arteriovenous fistula in the mouse are discussed. Under general anesthesia, an abdominal incision is made, and the aorta and inferior vena cava (IVC) are exposed. The proximal infrarenal aorta and the distal aorta are dissected for clamp placement and needle puncture, respectively. Special attention is paid to avoid dissection between the aorta and the IVC. After clamping the aorta, a 25 G needle is used to puncture both walls of the aorta into the IVC. The surrounding connective tissue is used for hemostatic compression. Successful creation of the AVF will show pulsatile arterial blood flow in the IVC. Further confirmation of successful AVF can be achieved by post-operative Doppler ultrasound.

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