Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 Jun;122(3):1017-24.
doi: 10.1128/jb.122.3.1017-1024.1975.

L-Asparaginase of Saccharomyces cerevisiae: an extracellular Enzyme

P C Dunlop, R J Roon

L-Asparaginase of Saccharomyces cerevisiae: an extracellular Enzyme

P C Dunlop et al. J Bacteriol. 1975 Jun.

Abstract

During recent studies conducted with suspensions of three strains of Saccharomyces cerevisiae, it was observed that ammonia was rapidly liberated when L-asparagine was added to the medium. Subsequent investigation has revealed that these strains of S. cerevisiae have an externally active asparaginase as well as an internally active one. The appearance of the external asparaginase is stimulated by nitrogen starvation, requires an available energy source, and is prevented by cycloheximide. The internal enzyme appears to be constitutive. The external activity is relatively insensitive to para-hydroxymercuribenzoate inhibition, whereas the internal activity is highly inhibited by this compound.

PubMed Disclaimer

References

    1. Eur J Biochem. 1973 May 2;34(3):479-82 - PubMed
    1. Biochim Biophys Acta. 1965 Jul 29;105(1):193-5 - PubMed
    1. Mol Gen Genet. 1973;121(1):9-14 - PubMed
    1. Adv Enzymol Relat Areas Mol Biol. 1973;39:185-248 - PubMed
    1. Biochem Genet. 1973 Jun;9(2):131-46 - PubMed