An immunohistochemical study of the presence and distribution of neuronal and glial markers in simple testicular cysts in the ostrich (Struthio camelus)

Abstract

Simple testicular cysts are rare in birds and mammals. However, the condition has recently been reported in the ostrich (Struthio camelus), an economically important farmed bird. The innervation of normal and cryptorchid testes, unlike the simple testicular cysts of birds and mammals, has received considerable attention. This study, therefore, immunohistochemically demonstrates the presence and the general distribution pattern of neuronal and glial markers in the simple intratesticular cyst, and its associated structures, of fourteen adult ostriches using antibodies to neurofilament protein, S-100, neuron-specific enolase and protein gene product 9.5. The LSAB+ Kit(DakoCytomation, Denmark) immunostaining protocol was used in this study. The normal seminiferous peritubular tissue showed few or no immunoreactive nerve fibres. A greater density of neurofilament protein, S-100, neuron-specific enolase and protein gene product 9.5 immunopositive nerve fibres were observed in the tunica albuginea adjacent to the cyst, as well as in the peritubular connective tissue of cystic seminiferous tubules. In addition, the tunica adventitia of blood vessels within the interstitial space of the cystic seminiferous tubules displayed neurofilament protein, S-100 and protein gene product 9.5 immunoreactive nerve fibres of varying intensity and pattern. Protein gene product 9.5 immunostaining was also observed in the multinucleated giant cells of both the normal and the cystic seminiferous tubules. The cystic portion of the testis appears to have a richer innervation than the normal portion of the same testis. The richer innervation of simple testicular cysts in the ostrich is similar to that observed in the cryptorchid testis of mammals.