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. 1990 Jul;56(7):2268-70.
doi: 10.1128/aem.56.7.2268-2270.1990.

Chromium reduction in Pseudomonas putida

Y Ishibashi  1 , C Cervantes, S Silver
Affiliations

Chromium reduction in Pseudomonas putida

Y Ishibashi et al. Appl Environ Microbiol. 1990 Jul.

Abstract

Reduction of hexavalent chromium (chromate) to less-toxic trivalent chromium was studied by using cell suspensions and cell-free supernatant fluids from Pseudomonas putida PRS2000. Chromate reductase activity was associated with soluble protein and not with the membrane fraction. The crude enzyme activity was heat labile and showed a Km of 40 microM CrO4(2-). Neither sulfate nor nitrate affected chromate reduction either in vitro or with intact cells.

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