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. 1990 Jun;371(6):485-91.
doi: 10.1515/bchm3.1990.371.1.485.

Disulfide bridges of bovine spleen cathepsin B

M Baudys  1 , B Meloun, T Gan-Erdene, J Pohl, V Kostka
Affiliations

Disulfide bridges of bovine spleen cathepsin B

M Baudys et al. Biol Chem Hoppe Seyler. 1990 Jun.

Abstract

Bovine spleen cathepsin B contains 7 disulfide bridges. Cleavage of the enzyme with cyanogen bromide gives rise to a large and a small fragment. The former contains all disulfide bridges. Their arrangement was determined by analysis of amino-acid sequences and compositions of subfragments prepared by cleavage of the large cyanogen-bromide fragment with trypsin, chymotrypsin and the staphylococcal proteinase using specific methods for the detection of S-S-bonds. Disulfide bridges link together Cys14-Cys43, Cys26-Cys71, Cys62-Cys128, Cys63-Cys67, Cys100-Cys132, Cys108-Cys119 and Cys148-Cys252.

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