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Comparative Study
. 2013 Oct;51(10):3418-20.
doi: 10.1128/JCM.01359-13. Epub 2013 Jul 31.

Comparison of the FDA-approved CDC DENV-1-4 real-time reverse transcription-PCR with a laboratory-developed assay for dengue virus detection and serotyping

Jesse J Waggoner  1 , Janaki Abeynayake, Malaya K Sahoo, Lionel Gresh, Yolanda Tellez, Karla Gonzalez, Gabriela Ballesteros, Frances P Guo, Angel Balmaseda, Kumudu Karunaratne, Eva Harris, Benjamin A Pinsky
Affiliations
Comparative Study

Comparison of the FDA-approved CDC DENV-1-4 real-time reverse transcription-PCR with a laboratory-developed assay for dengue virus detection and serotyping

Jesse J Waggoner et al. J Clin Microbiol. 2013 Oct.

Abstract

Dengue virus (DENV) is the agent of the most common vector-borne disease worldwide. Using 199 clinical samples collected from Nicaragua and Sri Lanka, a laboratory-developed DENV multiplex real-time reverse transcription-PCR (rRT-PCR) proved more clinically sensitive than the FDA-approved CDC assay for DENV serotypes 1 to 4 when measured against a composite reference standard, with sensitivities of 97.4% versus 87.1%, respectively.

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References

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