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. 2013 Aug;108(5):665-7.
doi: 10.1590/0074-0276108052013021.

Severity of tegumentary leishmaniasis is not exclusively associated with Leishmania RNA virus 1 infection in Brazil

Luiza de Oliveira Ramos Pereira  1 , Ana Cláudia Maretti-Mira, Káris Maria Rodrigues, Rosimar Baptista Lima, Manoel Paes de Oliveira-Neto, Elisa Cupolillo, Claude Pirmez, Márcia Pereira de Oliveira
Affiliations

Severity of tegumentary leishmaniasis is not exclusively associated with Leishmania RNA virus 1 infection in Brazil

Luiza de Oliveira Ramos Pereira et al. Mem Inst Oswaldo Cruz. 2013 Aug.

Abstract

Leishmania RNA virus (LRV) has been shown to be a symbiotic component of Leishmania parasites in South America. Nested retro-transcription polymerase chain reaction was employed to investigate LRV1 presence in leishmaniasis lesions from Brazil. In endemic areas of Rio de Janeiro (RJ), no LRV1 infection was observed even with mucosal involvement. LRV1 was only detected in Leishmania (V.) guyanensis cutaneous lesions from the northern region, which were obtained from patients presenting with disease reactivation after clinical cure of their primary lesions. Our results indicated that the severity of leishmaniasis in some areas of RJ, where Leishmania (V.) brazi-liensis is the primary etiological agent, was not associated with Leishmania LRV1 infection.

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Figures

None
Presence of Leishmania RNA virus 1 in clinical samples. Representative nested retro-transcription polymerase chain reaction (PCR) ethidium bromide stained 2.5% agarose gel. Ladders are 50 bp (left) and 100 bp (right). A: first round PCR using pair 1 primers; B: second and nested round using pair 2 primers; -: negative control; +: positive control with Leishmania (V.) guyanensis (MHOM/BR/1989/IM3597) cDNA; -*: double negative control using first round negative control as template; Lanes 1-3: state of Rio de Janeiro (RJ)-recidivant localised cutaneous leishmaniasis; 4, 8, 9: state of Amazonas-localised cutaneous leishmaniasis (LCL); 5-7: RJ-LCL; 10: RJ-scar; 11: RJ-mucosal leishmaniasis.
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