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Comment
. 2013 Jun 11;110(24):E2147-8.
doi: 10.1073/pnas.1304591110.

Reply to Ramos-Silva et al.: Regarding coral skeletal proteome

Jeana L Drake, Tali Massa, Liti Haramaty, Ehud Zelzion, Debashish Bhattacharya, Paul G Falkowski
Comment

Reply to Ramos-Silva et al.: Regarding coral skeletal proteome

Jeana L Drake et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1.
Fig. 1.
SEM image of bleached S. pistillata skeleton fragment. No residual surficial protein was observed on any of several skeleton fragments. (Scale bar, 1 μm.) (Inset) An EDS spectrum obtained from the center of this image of the fragment. Au peak is from gold coating. Similar spectra (all lacking nitrogen peaks that would be indicative of tissue contamination) were observed from several skeleton fragments. Images reproduced from ref. (figure 3C).
Fig. 2.
Fig. 2.
Silver-stained SDS/PAGE of SDS-soaked sub–150-μm S. pistillata skeleton powder. Powder was generated as described above, then soaked for two days in 2% (wt/vol) SDS at 4 °C. The soluble fraction was exchanged for new 2% (wt/vol) SDS after 2 d, and again after an additional 2 d. All solutions were concentrated by filter-centrifugation in 10-kDa Amicons, rinsed with PBS, and stored at −80 °C. All three rinsed and concentrated soluble fractions from each powder sample were combined, lyophilized, then resuspended and analyzed by SDS/PAGE on a 4–20% Tris⋅HCl Ready Gels (Bio-Rad) followed by silver staining (Pierce). Lane 1 is the molecular weight marker, lane 2 is solute from an S. pistillata obtained from an aquarium in the United States, and lane 3 is solute from a Red Sea S. pistillata. Staining of tops of lanes after overexposure (>10 min) of silver stain is normal. No bands were detected.
See this image and copyright information in PMC

Comment on

References

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