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. 1975 Aug;86(2):344-8.

Polymorphonuclear leukocyte phagocytosis: quantitation by a rapid radioactive method

D S Miller, S Beck
  • PMID: 239077

Polymorphonuclear leukocyte phagocytosis: quantitation by a rapid radioactive method

D S Miller et al. J Lab Clin Med. 1975 Aug.

Abstract

A rapid quantitative assay of phagocytosis has been developed. The method has been used to determine the phagocytic capability of human or dog polymorphonuclear leukocytes (PMN's) in a defined medium without added sera. The engulfment of 3H-labeled Staphylococcus albus is essentially complete in 30 minutes at 37 degrees C. Increase of phagocyte radioactivity is directly proportional to PMN per cubic centimeter over a wide range of cell concentrations. Maximal phagocytosis is observed at pH 7.5.

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