Studies of lactate dehydrogenase of Mycoplasma mycoides var. mycoides
- PMID: 239091
- DOI: 10.1099/00221287-88-1-58
Studies of lactate dehydrogenase of Mycoplasma mycoides var. mycoides
Abstract
Polyacrylamide gel electrophoresis and isoelectric focusing techniques have been used to compare NAD-dependent L(plus) lactate dehydrogenases (LDH) from ten different strains of Mycoplasma mycoides var. mycoides. The enzymes were not distinguished from one another, or from normal bovine LDH 1 by these methods. The kinetic behaviour of LDH form M. mycoides (T1 vaccine strain) suggested that the enzyme could readily reduce pyruvate or oxidize lactate in a manner which, in vertebrates, requires two different isoenzymes.
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