Cortisol enhances the stimulating action of thyroid hormones on dorsal fin-ray resorption of flounder larvae in vitro

Abstract

Effects of thyroid hormones and cortisol on fin-ray resorption of the larval flounder were studied in vitro. Dorsal fin rays were dissected from the prometamorphic larvae of the Japanese flounder (Paralichthys olivaceus) and were cultured for 7-8 days in a medium supplemented with different concentrations of thyroxine (T4) or triiodothyronine (T3) or combinations of either thyroid hormone or cortisol (0.1 micrograms/ml). Both T4 and T3 induced shortening of the second fin ray. T3 was more potent than T4; doses of 0.1 and 1.0 micrograms/ml T4 significantly accelerated shortening of the fin rays, whereas T3 was effective at concentrations ranging from 0.001 to 1.0 micrograms/ml. Cortisol further enhanced the effects of both T4 and T3 but was ineffective in the absence of thyroid hormones. When the prometamorphic larvae were reared in water containing T4 (0.01 micrograms/ml), T4 + cortisol (0.10 micrograms/ml), or cortisol alone for 15 days, both the T4- and T4 + cortisol-treated groups showed an advancement of the morphological changes associated with metamorphosis relative to the control group without hormone treatment. However, no clear synergistic effect of cortisol was observed in the in vivo experiment; a sufficient quantity of cortisol may be produced by the larval interrenal.