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. 2013 Jul;58(4):327.
doi: 10.4103/0019-5154.113959.

Expression of Inflammatory Biomarkers from Cultured Sebocytes was Influenced by Treatment with Vitamin D

Weon Ju Lee  1 , Yoon Hyuk Choi, Mi Yeung Sohn, Seok-Jong Lee, Do Won Kim
Affiliations

Expression of Inflammatory Biomarkers from Cultured Sebocytes was Influenced by Treatment with Vitamin D

Weon Ju Lee et al. Indian J Dermatol. 2013 Jul.

Abstract

Background: Inflammatory cytokines are the key factor in the pathophysiology of acne. It is well known that keratinocytes synthesize many kinds of inflammatory cytokines. In addition, it is reported that inflammatory cytokines are also expressed from sebocytes, which originate from the same stem cells with keratinocytes.

Aim: To clarify changes in the expression of inflammatory biomarkers from cultured sebocytes after treatment with vitamin D.

Materials and methods: Reverse transcription-polymerase chain reaction (RT-PCR) was done to measure changes in the expression of inflammatory biomarkers, including interleukin-1β (IL-1β), IL-6, IL-8, and tumor necrosis factor-α (TNF-α), and several subtypes of matrix metalloproteinases (MMPs) after treatment of a group of cultured sebocytes with vitamin D. Vitamin D receptor (VDR) small interfering RNA (siRNA) was added in the other group of cultured sebocytes to assure the role of vitamin D on the expression of inflammatory biomarkers. Enzyme-linked immunosorbent assay (ELISA) was also performed in the vitamin D-treated sebocytes.

Results: Cultured sebocytes showed non-significant changes in the gene expression of inflammatory biomarkers after treatment with vitamin D. In cultured sebocytes treated with a VDR siRNA, the expression of inflammatory biomarkers was not blocked after treatment with vitamin D. ELISA showed a significant decrease in the expression of IL-6, IL-8, and MMP-9, but a significant increase in the expression of MMP-1 and MMP-3, after treatment with vitamin D (10(-6) M).

Conclusion: Expression of inflammatory biomarkers is influenced by treatment with vitamin D in cultured sebocytes, but not through VDR.

Keywords: Acne; cultured sebocyte; inflammatory cytokines; vitamin D; vitamin D siRNA.

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Conflict of interest statement

Conflict of Interest: Nil.

Figures

Figure 1
Figure 1
The effect of vitamin D on the gene expression of IL-1 b (a), IL - 6 (b), IL - 8 (c), and TNF -α (d) in cultured sebocytes
Figure 2
Figure 2
ELISA of IL-1 b (a), IL - 6 (b), IL - 8 (c), and TNF -α (d) in cultured sebocytes
Figure 3
Figure 3
Effect of VDR siRNA on the gene expression of IL-1 b (a), IL - 6 (b), IL - 8 (c), and TNF -α (d) in cultured sebocytes after treatment with vitamin D
Figure 4
Figure 4
Effect of vitamin D on the gene expression of MMP-1 (a), MMP-3 (b), and MMP-9 (c) in cultured sebocytes
Figure 5
Figure 5
ELISA of MMP-1 (a), MMP-3 (b), and MMP-9 (c) in cultured sebocytes
Figure 6
Figure 6
Effect of VDR siRNA on the gene expression of MMP-1 (a), MMP-3 (b), and MMP-9 (c) in cultured sebocytes after treatment with vitamin D
See this image and copyright information in PMC

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