Sealing Ability of Orthograde MTA and CEM Cement in Apically Resected Roots Using Bacterial Leakage Method

Abstract

Introduction: The aim of this in vitro study was to determine the sealing ability of orthograde ProRoot mineral trioxide aggregate (MTA) and calcium enriched mixture (CEM) cement as root-end filling materials.

Materials and methods: Fifty four extracted single-rooted human teeth were used. The samples were randomly divided into 3 experimental groups. In group A and B, 4 mm of WMTA and CEM cement were placed in an orthograde manner and 3 mm of apices were resected after 24 hours. In group C the apical 3 mm of each root was resected and the root-end prepared with ultrasonic tips to a depth of 3 mm and subsequently, then filled with MTA. The apical sealing ability was performed with bacterial leakage method. Statistical analysis was carried out with Chi-square test.

Results: There were no significant differences in the extent of bacterial leakage between the three experimental groups (P>0.05).

Conclusion: Based on the limitations of this in vitro study, we concluded that MTA and CEM cement can be placed in an orthograde manner when there is a potential need for root-end surgery.

Keywords: Apicoectomy; Bacterial Leakage; CEM cement; Calcium Enriched Mixture; Mineral Trioxide Aggregate; Retrograde Obturation; Root Canal Filling Materials; Seal.

Figure 1.. Samples showed broth turbidity 90 days after canal filling with MTA and CEM

Figure 2.. Data distributions of survival test in experimental groups