doi: 10.1016/j.jbiosc.2013.06.004.
Epub 2013 Aug 6.
Increase in stearidonic acid by increasing the supply of histidine to oleaginous Saccharomyces cerevisiae
Affiliations
- PMID: 23932357
- DOI: 10.1016/j.jbiosc.2013.06.004
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Increase in stearidonic acid by increasing the supply of histidine to oleaginous Saccharomyces cerevisiae
J Biosci Bioeng.
2014 Jan.
Abstract
Increasing concentration of histidine significantly increased stearidonic acid production and cell growth in oleaginous Saccharomyces cerevisiae that has been genetically modified by Δsnf2 disruption, DGA1 and Δ6 desaturase gene overexpression, and LEU2 expression. High concentration of histidine in wild-type transformant and HIS3 expression in Δsnf2 transformant also increased stearidonic acid.
Keywords: DGA1; Histidine; Polyunsaturated fatty acid; Stearidonic acid; Δ6 Desaturase; Δsnf2; α-Linolenic acid.
Copyright © 2013 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
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