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. 2013 Nov 15;442(2):127-9.
doi: 10.1016/j.ab.2013.07.046. Epub 2013 Aug 8.

Cross-platform compatibility of Hi-Plex, a streamlined approach for targeted massively parallel sequencing

Tú Nguyen-Dumont  1 , Bernard J Pope, Fleur Hammet, Maryam Mahmoodi, Helen Tsimiklis, Melissa C Southey, Daniel J Park
Affiliations

Cross-platform compatibility of Hi-Plex, a streamlined approach for targeted massively parallel sequencing

Tú Nguyen-Dumont et al. Anal Biochem. .

Abstract

Although per-base sequencing costs have decreased during recent years, library preparation for targeted massively parallel sequencing remains constrained by high reagent cost, limited design flexibility, and protocol complexity. To address these limitations, we previously developed Hi-Plex, a polymerase chain reaction (PCR) massively parallel sequencing strategy for screening panels of genomic target regions. Here, we demonstrate that Hi-Plex applied with hybrid adapters can generate a library suitable for sequencing with both the Ion Torrent and the TruSeq chemistries and that adjusting primer concentrations improves coverage uniformity. These results expand Hi-Plex capabilities as an accurate, affordable, flexible, and rapid approach for various genetic screening applications.

Keywords: Disease gene screening; High-Plex PCR; Massively parallel sequencing; Molecular diagnostics; Targeted sequencing.

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References

    1. Nguyen-Dumont T, Pope BJ, Hammet F, Southey MC, Park DJ. A High-Plex PCR approach for massively parallel sequencing. BioTechniques. 2013;55:69–74. - PubMed
    1. Rahman N, Seal S, Thompson D, Kelly P, Renwick A, Elliott A, Reid S, Spanova K, Barfoot R, Chagtai T, Jayatilake H, McGuffog L, Hanks S, Evans DG, Eccles D, Easton DF, Stratton MR. PALB2, which encodes a BRCA2-interacting protein, is a breast cancer susceptibility gene. Nat. Genet. 2007;39:165–167. - PMC - PubMed
    1. Southey MC, Teo ZL, Dowty JG, Odefrey FA, Park DJ, Tischkowitz M, Sabbaghian N, Apicella C, Byrnes GB, Winship I, Baglietto L, Giles GG, Goldgar DE, Foulkes WD, Hopper JL. A PALB2 mutation associated with high risk of breast cancer. Breast Cancer Res. 2010;12:R109. - PMC - PubMed
    1. Park DJ, Lesueur F, Nguyen-Dumont T, Pertesi M, Odefrey F, Hammet F, Neuhausen SL, John EM, Andrulis IL, Terry MB, Daly M, Buys S, Le Calvez-Kelm F, Lonie A, Pope BJ, Tsimiklis H, Voegele C, Hilbers FM, Hoogerbrugge N, Barroso A, Osorio A, Giles GG, Devilee P, Benitez J, Hopper JL, Tavtigian SV, Goldgar DE, Southey MC. Rare mutations in XRCC2 increase the risk of breast cancer. Am. J. Hum. Genet. 2012;90:734–739. - PMC - PubMed

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