Leonidas Zervas award lecture: abiotic ligands for new quaternary architectures of peptides and proteins

Abstract

This review is the result of a kind invitation to provide a report on the basis of the Leonidas Zervas award lecture at the 32nd European Peptide Society in Athens, Greece, September 2012. The lecture covered several topics including contributions toward new methods for the assembly of peptides and chemical modification of proteins. The present review will focus on another topic from the Zervas lecture, namely, our recent efforts at creating new, artificial architectures for the organization of the quaternary structure of proteins. Crucially, this is achieved with abiotic ligands and not protein surface engineering. This concept was demonstrated for the important biopharmaceutical peptide insulin, by using both abiotic metal ion binding ligands and perfluoroalkyl chains.

Keywords: bipyridine; fluorous effect; metal ion binding ligands; peptide; perfluoroalkyl; protein; quaternary structure; small angle X-ray scattering.