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. 2013:8:2769-81.
doi: 10.2147/IJN.S45313. Epub 2013 Aug 2.

Zerumbone-loaded nanostructured lipid carriers: preparation, characterization, and antileukemic effect

Heshu Sulaiman Rahman  1 , Abdullah Rasedee, Chee Wun How, Ahmad Bustamam Abdul, Nazariah Allaudin Zeenathul, Hemn Hassan Othman, Mohamed Ibrahim Saeed, Swee Keong Yeap
Affiliations

Zerumbone-loaded nanostructured lipid carriers: preparation, characterization, and antileukemic effect

Heshu Sulaiman Rahman et al. Int J Nanomedicine. 2013.

Abstract

Zerumbone, a natural dietary lipophilic compound with low water solubility (1.296 mg/L at 25°C) was used in this investigation. The zerumbone was loaded into nanostructured lipid carriers using a hot, high-pressure homogenization technique. The physicochemical properties of the zerumbone-loaded nanostructured lipid carriers (ZER-NLC) were determined. The ZER-NLC particles had an average size of 52.68 ± 0.1 nm and a polydispersity index of 0.29 ± 0.004 μm. Transmission electron microscopy showed that the particles were spherical in shape. The zeta potential of the ZER-NLC was -25.03 ± 1.24 mV, entrapment efficiency was 99.03%, and drug loading was 7.92%. In vitro drug release of zerumbone from ZER-NLC was 46.7%, and for a pure zerumbone dispersion was 90.5% over 48 hours, following a zero equation. Using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay in human T-cell acute lymphoblastic leukemia (Jurkat) cells, the half maximal inhibitory concentration (IC50) of ZER-NLC was 5.64 ± 0.38 μg/mL, and for free zerumbone was 5.39 ± 0.43 μg/mL after 72 hours of treatment. This study strongly suggests that ZER-NLC have potential as a sustained-release drug carrier system for the treatment of leukemia.

Keywords: leukemia; nanostructured lipid carrier; zerumbone.

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Figures

Figure 1
Figure 1
Pure zerumbone crystals.
Figure 2
Figure 2
Appearance of zerumbone-loaded nanostructured lipid carriers.
Figure 3
Figure 3
Transmission electron microscopic image of zerumbone-loaded nanostructured lipid carriers.
Figure 4
Figure 4
Differential scan thermograms of (A) hydrogenated palm oil, (B) zerumbone, and (C) zerumbone-loaded nanostructured lipid carriers.
Figure 5
Figure 5
Powder x-ray diffraction patterns of (A) zerumbone, (B) hydrogenated palm oil, and (C) zerumbone-loaded nanostructured lipid carriers.
Figure 6
Figure 6
In vitro release profile of (A) zerumbone and (B) zerumbone from nanostructured lipid carriers.
Figure 7
Figure 7
Cytotoxicity assay of treated Jurkat cells with (A) zerumbone-loaded nanostructured lipid carriers, (B) zerumbone, and (C) blank nanostructured lipid carriers. Abbreviations: ZER, zerumbone; ZER-NLC, zerumbone-loaded nanostructured lipid carriers; h, hours.
See this image and copyright information in PMC

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