Microbial analysis in primary and persistent endodontic infections by using pyrosequencing

Abstract

Introduction: The aim of this study was to investigate the bacterial community profile of intracanal microbiota in primary and persistent endodontic infections associated with asymptomatic chronic apical periodontitis by using GS-FLX Titanium pyrosequencing. The null hypothesis was that there is no difference in diversity of overall bacterial community profiles between primary and persistent infections.

Methods: Pyrosequencing analysis from 10 untreated and 8 root-filled samples was conducted.

Results: Analysis from 18 samples yielded total of 124,767 16S rRNA gene sequences (with a mean of 6932 reads per sample) that were taxonomically assigned into 803 operational taxonomic units (3% distinction), 148 genera, and 10 phyla including unclassified. Bacteroidetes was the most abundant phylum in both primary and persistent infections. There were no significant differences in bacterial diversity between the 2 infection groups (P > .05). The bacterial community profile that was based on dendrogram showed that bacterial population in both infections was not significantly different in their structure and composition (P > .05).

Conclusions: The present pyrosequencing study demonstrates that persistent infections have as diverse bacterial community as primary infections.

Keywords: Diversity; operational taxonomic unit; persistent infection; primary infection; pyrosequencing; richness.