Cell separation of Tethya aurantia, an analytical study of embryonic and differentiated sponge cells

Abstract

The cells of the sponge Tethya aurantia var. californiana were separated on a Ficoll density gradient and the fractions analyzed for cell types and their lipids. Major cell types were choanocyte, archeocyte, and symbiont. Major differences in archeocyte and choanocyte fatty acid composition were noted for 20:4, 26:1 and 26:2. The fatty acids 26:1, 26:2, and 28:3 were dominant in the phosphatidylcholine fraction. Archeocytes had highest concentrations of 4,7,10,13-20:4 and 5,8,11,14-20:4 (arachidonic) acids which could be derived from symbionts, as odd-chain and methyl-branched fatty acid were also present. Sterol analyses showed cholesterol as a major sterol of the sponge cell fractions and clionasterol (or its 24-isomer) as a major sterol in symbiont cells.