A genetic variant in pre-miR-27a is associated with a reduced breast cancer risk in younger Chinese population

Abstract

Accumulating evidence has indicated that single nucleotide polymorphisms (SNPs) in miRNA precursors may have an effect on miRNA expression levels. Recently, an important A to G transition SNP in pre-mir-27a (rs895819) was identified. Previous studies have investigated the role of rs895819 in cancer risk, but the results remain contradictory, rather than conclusive. The present study aimed to evaluate the association of rs895819 with sporadic breast cancer susceptibility, clinicopathological characteristics and prognosis. We first analyzed the influence of rs895819 on the miR-27a expression level and determined that miR-27a expression was significantly lower in samples with the AG/GG genotype compared to samples with the AA genotype (p=0.022). Further investigation revealed that subjects with the G allele exhibited a significantly decreased risk of breast cancer relative to subjects carrying the A allele among the younger population (adjusted OR=0.628, 95% confidence interval 0.410-0.961; p=0.032). Moreover, significant associations between the presence of rs895819 and the histological grade and estrogen status of breast cancers were observed among the older group. However, the SNP did not influence the survival rate of patients with breast cancer. In conclusion, our results suggest that the SNP rs895819 may serve as a risk factor for breast cancer in younger Chinese populations; however, larger population-based studies are needed to validate these findings.

Keywords: Breast cancer; ER; HER2; HWE; Hardy-Weinberg equilibrium; IHC; Immunohistochemistry; PCR; PR; Polymerase chain reaction; Prognosis; SNP; Susceptibility; estrogen receptor; human epidermal growth factor receptor 2; miR-27a; progestogen receptor; rs895819; single nucleotide polymorphisms (SNPs).