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. 2013 Oct;10(10):981-4.
doi: 10.1038/nmeth.2603. Epub 2013 Aug 18.

Site-specific characterization of the Asp- and Glu-ADP-ribosylated proteome

Yajie Zhang  1 , Jianqi Wang, Ming Ding, Yonghao Yu
Affiliations

Site-specific characterization of the Asp- and Glu-ADP-ribosylated proteome

Yajie Zhang et al. Nat Methods. 2013 Oct.

Abstract

Poly(ADP-ribosyl)ation is catalyzed by a family of enzymes known as PARPs. We describe a method to characterize the human aspartic acid- and glutamic acid-ADP-ribosylated proteome. We identified 1,048 ADP-ribosylation sites on 340 proteins involved in a wide array of nuclear functions; among these were many previously unknown PARP downstream targets whose ADP-ribosylation was sensitive to PARP inhibitor treatment. We also confirmed that iniparib had a negligible effect on PARP activity in intact cells.

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