Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Jan;19(1):145-53.
doi: 10.1007/s12192-013-0452-9.

Reaction of small heat-shock proteins to different kinds of cellular stress in cultured rat hippocampal neurons

Britta Bartelt-Kirbach, Nikola Golenhofen

Reaction of small heat-shock proteins to different kinds of cellular stress in cultured rat hippocampal neurons

Britta Bartelt-Kirbach et al. Cell Stress Chaperones. 2014 Jan.

Erratum in

Abstract

Upregulation of small heat-shock proteins (sHsps) in response to cellular stress is one mechanism to increase cell viability.We previously described that cultured rat hippocampal neurons express five of the 11 family members but only upregulate two of them (HspB1 and HspB5) at the protein level after heat stress. Since neurons have to cope with many other pathological conditions, we investigated in this study the expression of all five expressed sHsps on mRNA and protein level after sublethal sodium arsenite and oxidative and hyperosmotic stress. Under all three conditions, HspB1, HspB5, HspB6, and HspB8 but not HspB11 were consistently upregulated but showed differences in the time course of upregulation. The increase of sHsps always occurred earlier on mRNA level compared with protein levels. We conclude from our data that these four upregulated sHsps (HspB1, HspB5, HspB6, HspB8) act together in different proportions in the protection of neurons from various stress conditions.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Changes of mRNA amount in cultured rat hippocampal neurons after different kinds of stress
Fig. 2
Fig. 2
Changes of protein amount in rat hippocampal neurons after different kinds of stress
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Bechtold DA, Brown IR. Heat shock proteins Hsp27 and Hsp32 localize to synaptic sites in the rat cerebellum following hyperthermia. Brain Res Mol Brain Res. 2000;75:309–320. doi: 10.1016/S0169-328X(99)00323-X. - DOI - PubMed
    1. Bellyei S, Szigeti A, Pozsgai E, Boronkai A, Gomori E, Hocsak E, Farkas R, Sumegi B, Gallyas F., Jr Preventing apoptotic cell death by a novel small heat shock protein. Eur J Cell Biol. 2007;86:161–171. doi: 10.1016/j.ejcb.2006.12.004. - DOI - PubMed
    1. Bjorkdahl C, Sjogren MJ, Zhou X, Concha H, Avila J, Winblad B, Pei JJ. Small heat shock proteins Hsp27 or alphaB-crystallin and the protein components of neurofibrillary tangles: tau and neurofilaments. J Neurosci Res. 2008;86:1343–1352. doi: 10.1002/jnr.21589. - DOI - PubMed
    1. Bonefeld BE, Elfving B, Wegener G. Reference genes for normalization: a study of rat brain tissue. Synapse. 2008;62:302–309. doi: 10.1002/syn.20496. - DOI - PubMed
    1. Boyd SD. Everything you wanted to know about small RNA but were afraid to ask. Lab Invest. 2008;88:569–578. doi: 10.1038/labinvest.2008.32. - DOI - PubMed