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. 2013 Nov;51(11):3839-40.
doi: 10.1128/JCM.01386-13. Epub 2013 Aug 21.

Development of a loop-mediated isothermal amplification assay targeting the mpb64 gene for diagnosis of intraocular tuberculosis

Praveen Kumar Balne  1 , Manas Ranjan Barik, Savitri Sharma, Soumyava Basu
Affiliations

Development of a loop-mediated isothermal amplification assay targeting the mpb64 gene for diagnosis of intraocular tuberculosis

Praveen Kumar Balne et al. J Clin Microbiol. 2013 Nov.

Abstract

A loop-mediated isothermal amplification (LAMP) assay targeting the mpb64 gene for the diagnosis of intraocular tuberculosis was highly specific (100%), sensitive (85.7%), rapid, and easy to perform. The LAMP assay can be an alternative to conventional PCR for the diagnosis of ocular tuberculosis in resource-limited settings.

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Figures

Fig 1
Fig 1
(a) Visual detection of LAMP-amplified products. Positive reactions indicated by a yellowish green color are seen in tubes S1, S2, and S3 (patient samples) and tube PC (positive control), while a reddish orange color indicates a negative reaction in tube NC (negative control). (b) Agarose gel electrophoresis (2%) picture of LAMP assays showing DNA amplification (ladder-like banding pattern) in lanes S1, S2, and S3 (patient samples) and lane PC (positive control). No amplification was detected in lane NC (negative control). MW (molecular weight), 100-bp DNA ladder.
See this image and copyright information in PMC

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