Angiotensin- converting enzyme insertion/deletion polymorphism and its association with coronary artery disease in an Iranian population

Abstract

Background: The study of the association between genotype and phenotype is of great importance for the prediction of many diseases and pathophysiological conditions. The relationship between angiotensin-converting enzyme (ACE) gene insertion/ deletion (I/D) polymorphism and pathological processes such as coronary artery disease (CAD) has been investigated previously with discordant results. This study was designed to determine the association between ACE gene I/D polymorphism and CAD in an Iranian population.

Methods: A total of 1050 individuals who were referred to Tehran Heart Center for coronary angiography were recruited. Six hundred seventy-six CAD-positive patients (documented by coronary angiography and Gensini scores higher than 6) and 374 CAD-negative patients were evaluated for ACE gene I/D polymorphism via the Polymerase Chain Reaction Amplification method. The patients' age, sex, smoking status and its duration as well as familial history of CAD, hypertension, and diabetes mellitus were recorded.

Results: Five hundred four (74.6%) of the CAD-positive patients were male, and the mean age of this group was 60 (60 ± 10). In the CAD-negative individuals, the mean age was 56 (56 ± 10) and 196 of them were male (52.4%). After the analysis of all the groups and gender subgroups, neither genotype nor allele frequency was significantly different between the CAD-positive and CAD-negative groups (p values for genotypes and allele frequencies were 0.494 and 0.397, respectively).

Conclusion: ACE gene I/D polymorphism was not associated with an increased risk of CAD in an Iranian population.

Keywords: Coronary artery disease; Iran; Polymorphism, genetic.

Figure 1

Gel electrophoresis image showing results of Angiotensin-converting enzyme Insertion/deletion polymorphism. Lines 1, 2, 10 and 12 represent normal homozygote patients, lines 5, 6 and 9 represent mutant homozygote patients and lines 3, 4, 7 and 8 represent heterozygote patients. Ladder 50bp (CinnaGen, Iran)