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. 2013 Jun 1;42(6):620-5.
eCollection 2013.

Molecular Genotyping of Toxoplasma gondii in Human Spontaneous Aborted Fetuses in Shiraz, Southern Iran

Qasem Asgari  1 , Maysam Fekri, Ahmad Monabati, Mohsen Kalantary, Iraj Mohammadpour, Mohammad Hossein Motazedian, Bahador Sarkari
Affiliations

Molecular Genotyping of Toxoplasma gondii in Human Spontaneous Aborted Fetuses in Shiraz, Southern Iran

Qasem Asgari et al. Iran J Public Health. .

Abstract

Background: Congenital toxoplasmosis is associated with variable complications including encephalitis, microcephaly, hydrocephaly, hepatitis, lymphadenopathy and even intrauterine death. Presence of Toxoplasma gondii in human placenta may induce congenital infection. The aim of this study was to determine the genotypes of Toxoplasma gondii infection in human spontaneous aborted fetuses in Shiraz, south of Iran.

Methods: Five hundred and forty two paraffin-embedded blocks of aborted placenta were collected, from two university-affiliated hospitals in Shiraz. Occurrence of spontaneous abortion was confirmed by examine of the slides. After re-cutting of the blocks and dewaxing, semi-nested PCR assay was used to detect the fragments of T. gondii B1 gene in the samples. Also direct molecular genotyping was performed on positive samples with Restriction Fragment Length Polymorphism-PCR analysis on the SAG2 gene.

Results: Among the 542 tissue samples, the B1 gene was amplified from 78 (14.4%) of cases with the semi nested PCR and typed by RFLP. The genotype of Toxoplasma strains of 65 (out of 78) PCR-positive samples were evaluated and 54 out of 65 (83.1%) were found to be type II and 11 out of 65 (16.9%) were type I.

Conclusion: Considering the high level of Toxoplasma infection in aborted fetuses in this study, Toxoplasma might largely contribute to spontaneous abortion in this area of Iran.

Keywords: Abortion; Genotype; Iran; Toxoplasma gondii.

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Figures

Fig. 1:
Fig. 1:
The amplicons produced, in the semi Nested PCR based on the B1 gene primers, from aborted placenta. The amplified product of 183 bp belonged to Toxoplasma. /Lane 1–18: positive tissue of placenta; lane 19: negative sample, lane 20: positive sample (Toxoplasma gondii RH strain), Lane 21: DNA ladder
Fig. 2:
Fig. 2:
Restriction enzyme analysis of PCR products from Toxoplasma positive aborted tissues digested with HhaI. Lane 1: 100 bp DNA ladder; Lane 1–7 and 9–11: type II of Toxoplasma, lane 8: type 1, lane 12: positive control (type 1; RH strain)
See this image and copyright information in PMC

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