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. 1975 Feb;145(2):323-34.
doi: 10.1042/bj1450323.

The purification of fructose 1,6-diphosphatase from ox liver and its activation by ethylenediaminetetra-acetate

H G Nimmo, K F Tipton

The purification of fructose 1,6-diphosphatase from ox liver and its activation by ethylenediaminetetra-acetate

H G Nimmo et al. Biochem J. 1975 Feb.

Abstract

1.A procedure for the purification of ox liver fructose 1,6-kiphosphatase is described. A number of criteria indicate that the enzyme was not subjected to any significant degree of proteolytic attack during the purification. 2. The molecular weight, amino acid composition and subunit molecular weight are reported. 3. The activation by EDTA was shown to be due to the chelation heavy metals rather than by a more complex interaction with the enzyme as had previously been suggested.

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