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. 1975 Apr;147(1):103-9.
doi: 10.1042/bj1470103.

Purification and heterogeneity of inorganic pyrophosphatase of pig scapula cartilage

R Felix, H Fleisch

Purification and heterogeneity of inorganic pyrophosphatase of pig scapula cartilage

R Felix et al. Biochem J. 1975 Apr.

Abstract

Inorganic pyrophosphatase (pyrophosphate phosphohydrolase; EC 3.6.1.1) was purified from pig scapula cartilage by fractionation with N-cetylpyridinium chloride and (NH4)2SO4, followed by ion-exchange and gel-filtration chromatography. Enzyme preparations of high purity were obtained, with specific activities (100-400 units/mg) higher than those previously described for mammalian pyrophosphatases. The enzyme activity could be separated into several subfractions on ion-exchange columns.

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