The formation of mono-N-acetylhexosamine derivatives of dolichol diphosphate by pig liver microsomal fractions
- PMID: 239708
- PMCID: PMC1165532
- DOI: 10.1042/bj1480245
The formation of mono-N-acetylhexosamine derivatives of dolichol diphosphate by pig liver microsomal fractions
Abstract
Incubation of pig liver microsomal preparations with UDP-N[U-14C]acetylglucosamine yields a 14C-labelled lipid. The requirement for Mn2+, the pH optimum, time-dependence and the reversibility by UMP of the transferase are reported. Evidence is presented in favour of the lipid being a mixture of dolichol diphosphate N-[14C]acetylglucosamine and dolichol diphosphate N-[14C]acetylmannosamine. Available data suggest that the epimerization takes place while the hexosamine is bound in this lipid-soluble form. The N-acetylmannosamine appeared not be be released into the medium. The subfractionation of the microsomal fraction to separate transferase activity from membrane-bound beta-N-acetylglucosaminidase activity is also reported.
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