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Multicenter Study
. 2013 Dec;29(12):1589-93.
doi: 10.1089/AID.2013.0097. Epub 2013 Oct 2.

Short communication: Evidence that microbial translocation occurs in HIV-infected children in the United Kingdom

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Multicenter Study

Short communication: Evidence that microbial translocation occurs in HIV-infected children in the United Kingdom

Felicity Fitzgerald et al. AIDS Res Hum Retroviruses. 2013 Dec.

Abstract

Microbial translocation (MT) from the gut is implicated in driving immune activation, increasing morbidity and mortality in HIV. We used bacterial 16S rDNA PCR, Sanger sequencing, and high-throughput sequencing to identify microbial DNA in the bloodstream of HIV-infected children in London, United Kingdom. Blood samples were collected from sequential children attending the HIV clinic at Great Ormond Street Hospital, London. DNA extraction, broad range 16S rDNA PCR, and standard Sanger sequencing were carried out. A subset of positive samples was analyzed by high-throughput sequencing (Roche 454 platform). Of 105 samples collected from sequential children, nine were positive using broad range 16S rDNA PCR (8.6%; 95% CI 4.4-16%). From three amplicons, 16S rDNA sequences were identified as Streptococcus, Propionibacterium acnes, and coagulase-negative Staphylococcus. Four positive samples were analyzed by high-throughput sequencing. In the three samples in which organisms were identified by Sanger sequencing, the same species were identified. Further species, in differing proportions, were identified in all four samples. The identified organisms included known gut orders Bifidobacteriaceae, Lactobacillaceae, Bacteroidales, and Clostridiales. In immunocompetent children of equivalent age, no bacterial DNA was detected in blood using this approach. This is the first study to our knowledge using molecular techniques to identify MT in children in the developed world. Our data indicate that 16S rDNA is detectable in 8.6% of HIV-infected children. Levels of DNA were low and from multiple bacterial species. Further studies are needed to ascertain the importance of MT in HIV-infected children.

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Figures

FIG. 1.
FIG. 1.
Bacteria orders recovered from HIV+ blood using 16S rDNA high-throughput sequencing. Proportions of the total recovered sequences from each sample after quality filtering and assigning taxonomy to reads generated by high-throughput sequencing. Samples are labeled A, B, C, and D.

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References

    1. Massanella M. Negredo E. Perez-Alvarez N, et al. CD4 T-cell hyperactivation and susceptibility to cell death determine poor CD4 T-cell recovery during suppressive HAART. AIDS. 2010;24(7):959–968. - PubMed
    1. Hunt PW. Martin JN. Sinclair E, et al. T cell activation is associated with lower CD4+ T cell gains in human immunodeficiency virus-infected patients with sustained viral suppression during antiretroviral therapy. J Infect Dis. 2003;187(10):1534–1543. - PubMed
    1. Bofill M. Mocroft A. Lipman M, et al. Increased numbers of primed activated CD8+CD38+CD45RO+ T cells predict the decline of CD4+ T cells in HIV-1-infected patients. AIDS. 1996;10(8):827–834. - PubMed
    1. Giorgi JV. Hultin LE. McKeating JA, et al. Shorter survival in advanced human immunodeficiency virus type 1 infection is more closely associated with T lymphocyte activation than with plasma virus burden or virus chemokine coreceptor usage. J Infect Dis. 1999;179(4):859–870. - PubMed
    1. Baker JV. Peng G. Rapkin J, et al. CD4+ count and risk of non-AIDS diseases following initial treatment for HIV infection. AIDS. 2008;22(7):841–848. - PMC - PubMed

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