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Comparative Study
. 2013 Nov;30(11):1505-12.
doi: 10.1007/s10815-013-0088-9. Epub 2013 Aug 25.

HOXA-11 mediated dysregulation of matrix remodeling during implantation window in women with endometriosis

Saikat Kumar Jana  1 , Priyanka Banerjee, Rashmi Mukherjee, Baidyanath Chakravarty, Koel Chaudhury
Affiliations
Comparative Study

HOXA-11 mediated dysregulation of matrix remodeling during implantation window in women with endometriosis

Saikat Kumar Jana et al. J Assist Reprod Genet. 2013 Nov.

Abstract

Purpose: To investigate the Homeobox genes HOXA-10 and HOXA-11 mediated endometrial molecular defects during implantation window in endometriosis-associated infertility cases.

Methods: Endometrial biopsies were obtained during implantation window from 31 infertile women with endometriosis (age < 35 years) and 26 age and BMI-matched infertile women without endometriosis were included in the study for comparison purposes. Endometrial expression of HOXA-10 and HOXA-11 genes, MMP-2, -9, α(v)β(3) integrin, leukemia inhibitory factor and surface characteristics including average roughness and topology were assessed.

Results: A significantly lower expression of HOXA-10 and HOXA-11 were observed in endometriotic women compared to non-endometriotic controls. Further, a significantly higher endometrial expression of MMP-2 and -9 were observed in women with endometriosis when compared with controls. Interestingly, endometrial surface were observed to be grossly affected in terms of average roughness and topology in women with endometriosis compared to controls.

Conclusions: The findings suggest that aberrant expression of HOXA-10 and -11 genes adversely affects endometrial remodelling and expression of receptivity markers.

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Figures

Fig. 1
Fig. 1
Graphical representation of mRNA expression of a MMP-2 b MMP-9 c HOXA-11 d HOXA-10 in the endometrium of women with endometriosis and controls. e Endometrial expression of MMP-2, MMP-9, HOXA-11, HOXA-10 and β-actin in women with endometriosis and controls. E: endometriosis and C: control
Fig. 2
Fig. 2
a, b, d, e Typical atomic force microscopic images of endometrial tissue: a 2D image of endometrial tissue of control b 3D image of endometrial tissue of control d 2D image of endometrial tissue of women with endometriosis e 3D image of endometrial tissue of women with endometriosis. c Typical height profile of endometrial tissue of control f Typical height profile of endometrial tissue of women with endometriosis
Fig. 3
Fig. 3
a-f Typical scanning electron microscopic images of pinopodes: a well developed b developing c absent d abundant e moderate f few. g-h graphical representation of pinopodes formation in women with endometriosis and controls
Fig. 4
Fig. 4
a-d Immunohistological images of different biochemical markers expression: a αvβ3 integrin in controls b αvβ3 integrin in women with endometriosis c LIF in controls d LIF in endometriosis. Graphical representation of e αvβ3 integrin expression in the stromal and epithelial cells of endometrial tissues in endometriosis and control f LIF expression in the stromal and epithelial cells of endometrial tissues in endometriosis and control
See this image and copyright information in PMC

References

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